1. Chinangwa: Maitiro aya ane chinangwa chekupa maitiro akajairika ekushanda kweaseptic uye kuchengetedza makamuri asina utachiona.
2. Mashandisirwo azvo: rabhoritari yekuyedza zvinhu zvipenyu
3. Munhu Ane Mutoro: Muongorori weQC
4.Tsananguro: Hapana
5. Matanho ekuchengetedza
Ita mabasa asina utachiona zvakanyanya kudzivirira kusvibiswa kwehutachiona; vashandi vanofanira kudzima rambi reUV vasati vapinda mukamuri risina utachiona.
6. Maitiro
6.1. Imba isina utachiona inofanira kunge ine imba yekuvhiyira isina utachiona uye imba yekuchengetera. Kuchena kweimba yekuvhiyira isina utachiona kunofanira kusvika pachikamu che10000. Tembiricha yemukati inofanira kuchengetedzwa pa20-24°C uye hunyoro hunofanira kuchengetedzwa pa45-60%. Kuchena kwebhenji rakachena kunofanira kusvika pachikamu che100.
6.2. Imba isina utachiona inofanira kucheneswa, uye zvinorambidzwa zvachose kuunganidza marara kudzivirira kusvibiswa.
6.3. Dzivisai kusvibiswa kwemidziyo yese yekuuraya utachiona uye nzira dzekudzikisa utachiona. Avo vane utachiona vanofanira kurega kuishandisa.
6.4. Mumba musina utachiona munofanira kunge muine mishonga inouraya utachiona inoshanda, yakaita se5% cresol solution, 70% alcohol, 0.1% chlormethionine solution, nezvimwewo.
6.5. Mumba musina utachiona munofanira kugara muchicheneswa nemishonga inouraya utachiona kuti muve nechokwadi chekuti kuchena kwemumba musina utachiona kunoenderana nezvinodiwa.
6.6. Midziyo yese, midziyo, ndiro nezvimwe zvinhu zvinoda kuunzwa muimba isina utachiona zvinofanirwa kuputirwa zvakasimba uye kucheneswa nemishonga nenzira dzakakodzera.
6.7. Vasati vapinda mukamuri risina utachiona, vashandi vanofanira kugeza maoko avo nesipo kana mushonga unouraya utachiona, vobva vapfeka mbatya dzebasa, shangu, ngowani, mamasiki nemagirovhosi muimba yekuchengetera (kana kupukuta maoko avo zvakare ne70% ethanol) vasati vapinda mukamuri risina utachiona. Vanofanira kuita oparesheni mukamuri rine utachiona.
6.8. Usati washandisa jira risina tsvina, rambi re ultraviolet riri mukamuri risina tsvina rinofanira kubatidzwa kuti rivhenekerwe uye richeneswe kweanopfuura maminetsi makumi matatu, uye bhenji rakachena rinofanira kubatidzwa kuti mweya ufuridzwe panguva imwe chete. Mushure mekunge basa rapera, jira risina tsvina rinofanira kucheneswa nenguva uye rozocheneswa nechiedza che ultraviolet kwemaminetsi makumi maviri.
6.9. Musati maongorora, chifukidziro chekunze chemuenzaniso wekuyedza chinofanira kuchengetwa chisina kusvibiswa uye hachifanirwe kuvhurwa kudzivirira kusvibiswa. Musati maongorora, shandisai mabhora edonje ane alcohol 70% kuti muuraye pamusoro pekunze.
6.10. Pakuvhiya kwega kwega, kudzora negative kunofanirwa kuitwa kuti vaone kuvimbika kwekushanda kweaseptic.
6.11. Paunenge uchisveta mvura inobuda mubhakitiriya, unofanira kushandisa bhora rekusveta kuti uisvete. Usabata tsvina yacho zvakananga nemuromo wako.
6.12. Tsono yekubaiwa inofanira kupiswa nemoto usati washandisa uye mushure mekushandisa kwega kwega. Mushure mekutonhodza, mbeu inogona kubaiwa.
6.13. Mashanga, machubhu ekuyedza, ndiro dzepetri nezvimwe midziyo ine mvura inouraya utachiona zvinofanirwa kunyikwa mubhaketi rekuuraya utachiona rine 5% Lysol solution yekuuraya utachiona, uye zvobviswa zvosukwa mushure memaawa makumi maviri nemana.
6.14. Kana paine mvura inobuda mubhakitiriya yakadururwa patafura kana pasi, unofanira kudurura 5% carbolic acid solution kana 3% Lysol panzvimbo ine utachiona nekukurumidza kwemaminitsi makumi matatu usati wairapa. Kana mbatya dzebasa nengowani zvasvibiswa nemvura inobuda mubhakitiriya, zvinofanira kubviswa nekukasika zvogezwa mushure mekupiswa nemvura ine simba rakawanda.
6.15. Zvinhu zvese zvine mabhakitiriya mapenyu zvinofanirwa kucheneswa nehutachiona zvisati zvasukwa pasi pepombi. Zvinorambidzwa zvachose kusvibisa tsvina yetsvina.
6.16. Huwandu hwemakoroni ari muimba isina utachiona hunofanira kuongororwa mwedzi wega wega. Kana bhenji rakachena rakavhurika, tora ndiro dzakawanda dzepetri dzisina utachiona dzine dhayamita yemukati ye90 mm, woisa 15 ml ye nutritive agar culture medium yakanyungudutswa uye yakatonhodzwa kusvika pa45°C. Mushure mekuoma, isa yakatarisa pasi pa30 kusvika 35 Incubate kwemaawa 48 mu ℃ incubator. Mushure mekuratidza kusaoma, tora ndiro 3 kusvika 5 wodziisa kuruboshwe, pakati nerudyi rwenzvimbo yekushanda. Mushure mekuvhura chifukidziro nekudziisa pachena kwemaminitsi 30, dziise yakatarisa pasi mu 30 kusvika 35°C incubator kwemaawa 48 wodziburitsa. ongorora. Avhareji yehuwandu hwemabhakitiriya akasiyana-siyana ari mundiro munzvimbo yakachena yekirasi 100 haizopfuuri koloni imwe chete, uye avhareji yenhamba muimba yakachena yekirasi 10000 haifaniri kudarika koloni 3. Kana muganho wacho ukadarika, imba isina utachiona inofanira kucheneswa zvakakwana kusvika kuongororwa kwakaitwa kakawanda kwazadzisa zvinodiwa.
7. Tarisa chitsauko (Nzira yekuongorora kusabereka) mu "Nzira dzekuongorora hutsanana hwemishonga" uye "China Standard Operating Practices for Drug Inspection".
8. Dhipatimendi reKugovera: Dhipatimendi reKutarisira Hunhu
Gwaro rehunyanzvi rekuchenesa imba:
Mushure mekuwana nharaunda isina utachiona uye zvinhu zvisina utachiona, tinofanira kuchengetedza mamiriro asina utachiona kuitira kuti tidzidze tupukanana twatinozivikanwa kana kushandisa mabasa atwo. Zvikasadaro, tupukanana twakasiyana-siyana tunobva kunze tunogona kusanganisa zviri nyore. Chiitiko chekusanganiswa kwetupukanana tusina basa kubva kunze chinonzi mabhakitiriya anosvibisa mutupukanana. Kudzivirira kusvibiswa inzira yakakosha mubasa retupukanana. Kuchenesa zvakakwana kune rumwe rutivi uye kudzivirira kusvibiswa kune rumwe rutivi zvinhu zviviri zvemaitiro easeptic. Pamusoro pezvo, tinofanira kudzivirira tupukanana turi kudzidzwa, kunyanya tupukanana tunokonzera zvirwere kana tupukanana twakagadzirwa nemajini tusipo muzvisikwa, kuti tisabude mumidziyo yedu yekuedza kuenda kune imwe nzvimbo. Pazvinangwa izvi, mutupukanana, kune matanho akawanda.
Kamuri risina utachiona rinowanzova kamuri kadiki kakagadzirwa murabhoritari ye microbiology. Rinogona kuvakwa nemachira negirazi. Nzvimbo yacho haifanirwe kunge yakakura zvakanyanya, inosvika 4-5 square metres, uye kureba kwayo kunofanira kunge kuri 2.5 metres. Kamuri rekuchengetedza rinofanira kuiswa kunze kwekamuri risina utachiona. Musuwo wekamuri rekuchengetedza mweya nemusuwo wekamuri risina utachiona hazvifanirwe kutarisana nedivi rimwe chete kudzivirira kuyerera kwemhepo kubva pakuunza mabhakitiriya akasiyana-siyana. Kamuri rese risina utachiona nemukamuri rekuchengetedza mweya zvinofanira kunge zvisina mhepo. Midziyo yekufambisa mweya yemukati inofanira kunge iine michina yekusefa mweya. Pasi nemadziro ekamuri risina utachiona zvinofanira kunge zvakapfava, zvakaoma kuchengetedza tsvina uye zviri nyore kuchenesa. Nzvimbo yebasa inofanira kunge yakaenzana. Kamuri rese risina utachiona nemukamuri rekuchengetedza mweya zvine mwenje ye ultraviolet. Mwenje ye ultraviolet mukamuri risina utachiona iri kure nemita imwe kubva panzvimbo yebasa. Vashandi vanopinda mukamuri risina utachiona vanofanira kupfeka mbatya dzakachena nengowani.
Parizvino, makamuri asina tsvina anowanikwa mumafekitori e microbiology, nepo marabhoritari ese achishandisa bhenji rakachena. Basa guru rebhenji rakachena nderekushandisa mudziyo we laminar air flow kubvisa guruva diki diki kusanganisira tupukanana panzvimbo yebasa. Mudziyo wemagetsi unobvumira mweya kupfuura nepa hepa filter wozopinda panzvimbo yebasa, kuitira kuti nzvimbo yebasa igare ichidzorwa nemhepo isina tsvina inoyerera. Uyezve, kune keteni remhepo rinomhanya zvakanyanya parutivi rwuri pedyo nekunze kudzivirira mhepo yekunze yebhakitiriya kuti isapinde.
Munzvimbo dzine mamiriro akaoma, mabhokisi emapuranga asina utachiona anogona kushandiswawo panzvimbo pebhenji rakachena. Bhokisi risina utachiona rine chimiro chakareruka uye riri nyore kufambisa. Kune maburi maviri pamberi pebhokisi, ayo anovharwa nemasuo ekudhonza kana asiri kushanda. Unogona kutambanudza maoko ako mukati panguva yekushanda. Chikamu chepamusoro chepamberi chakashongedzwa negirazi kuti zviite kuti kushanda kwemukati kushande. Mune rambi reultraviolet mukati mebhokisi, uye midziyo nemabhakitiriya zvinogona kuiswa nepamusuwo mudiki padivi.
Matekiniki ekushandisa anodzivirira utachiona pari zvino haangoite basa guru mukutsvaga nekushandisa ma microbiological chete, asiwo anoshandiswa zvakanyanya mu biotechnologies dzakawanda. Semuenzaniso, transgenic technology, monoclonal antibody technology, nezvimwewo.
Nguva yekutumira: Kurume-06-2024